技术领域
本发明涉及癫痫诊断领域，具体而言，涉及一种辅助癫痫诊断的标志物及其检测试剂盒。
背景技术
癫痫是慢性反复发作性短暂脑功能失调综合征。以脑部神经元高度同步化异常放电引起反复痫性发作为特征。癫痫是神经系统常见疾病之一，患病率仅次于脑卒中。癫痫的发病率与年龄有关。一般认为1岁以内患病率最高，1～10岁以后逐渐降低。我国癫痫患者男女之比为(1.15-1.7):1。
癫痫病因极其复杂，可分三大类，并存在多种影响发病的因素：
1.特发性癫痫
可疑遗传倾向且无其他明显病因，常在特定年龄段起病，有特征性临床及脑电图表现，诊断较明确。
2.症状性癫痫
中枢神经系统病变影响结构或功能等，如染色体异常、局灶性或弥漫性脑部疾病，以及某些系统性疾病所致。
(1)局限性或弥漫性脑部疾病①先天性异常胚胎发育中各种病因导致脑穿通畸形、小头畸形、先天性脑积水胼胝体缺如及大脑皮质发育不全，围生期胎儿脑损伤等；②获得性脑损伤如脑外伤，颅脑手术后，脑卒中后，颅内感染后，急性酒精中毒；③产伤所致新生儿癫痫发生率约为1％，分娩时合并产伤多伴脑出血或脑缺氧损害，新生儿合并脑先天发育畸形或产伤，癫痫发病率高达25％；④感染包括中枢神经系统细菌、病毒、真菌、寄生虫、螺旋体感染及AIDS神经系统并发症等；⑤脑血管疾病如脑动静脉畸形、脑梗死和脑出血等；⑥颅内原发性肿瘤如神经胶质瘤、脑膜瘤等；⑦遗传代谢性疾病如结节性硬化、脑-面血管瘤病、苯丙酮酸尿症等；⑧神经系统变性疾病如Alzheimer病、Pick病等约1/3的患者合并癫痫发作。
(2)系统性疾病①缺氧性脑病如心搏骤停、CO中毒窒息、麻醉意外和呼吸衰竭等可引起肌阵挛性发作或全身性大发作；②代谢性脑病如低血糖症，其他代谢及内分泌障碍如高血糖症、低钙血症、低钠血症，以及尿毒症、肝性脑病和甲状腺毒血症等均可导致癫痫发作；③心血管疾病如心脏骤停、高血压脑病等；④热性惊厥热性发作；⑤子痫；⑥中毒如酒精、异烟肼、卡巴唑等药物及铅、铊等重金属中毒。
3.隐源性癫痫
较多见，临床表现提示症状性癫痫，但未找到明确病因，可在特殊年龄段起病，无特定临床和脑电图表现。
癫痫的临床表现有以下：
1.全面强直-阵挛发作(大发作)
系指全身肌肉抽动及意识丧失的发作。以产伤、脑外伤、脑瘤等较常见。强直-阵挛发作可发生在任何年龄，是各种癫痫中最常见的发作类型。其典型发作可分为强直期、阵挛期、恢复期三个临床阶段。发作期间脑电图为典型的爆发性多棘波和棘-慢波综合，每次棘-慢波综合可伴有肌肉跳动。
2.单纯部分发作
是指脑的局部皮质异常放电而引起的与该部位的功能相对应的症状，发作时意识始终存在，包括运动、感觉、自主神经、精神症状及体征。分为四组：①伴运动症状者；②伴躯体感觉或特殊感觉症状者；③伴自主神经症状和体征者；④伴精神症状者。
3.复杂部分发作
习惯上又称精神运动性发作，伴有意识障碍。先兆多在意识丧失前或即将丧失时发生，发作后不能或部分不能回忆发作细节。4.失神发作(小发作)
其典型表现为意识障碍，而不伴先兆或发作后症状。
5.癫痫持续状态
是指单次癫痫发作超过5分钟，或者短期内癫痫频繁发作，以致患者尚未从前一次发作中完全恢复而又有另一次发作。癫痫持续状态是一种需要抢救的急症。
癫痫的治疗可分为控制发作、病因治疗、外科治疗、一般卫生及预防五个方面。其中最重要的是控制发作，目前以药物治疗为主。
临床上根据癫痫发作类型选用抗癫痫药物，一旦找到可以完全控制发作的药物和剂量，就应不间断地应用。一般应于发作完全控制后，如无不良反应再继续服用3～5年，方可考虑逐渐停药。目前多主张用单药治疗，确认单药治疗失败后，方可加用第2种药物。如失神发作或肌阵挛发作无法用单药控制者，可合用乙琥胺和丙戊酸钠，或其一加用苯二氮类。对合并多种发作类型者可以根据发作类型合理联合用药，但以不超过3种药物为宜。
用药宜从小剂量开始，然后逐渐增量，以既能控制发作，又不产生毒性反应的最小有效剂量为宜。换药宜采取先加用新药及后递减旧药的原则。不能骤然停药。
有些器质性脑病的癫痫患者可能需要终身服药；有人主张发病年龄大于30岁者需谨慎停药，因其停药后复发率较高，需长期服药或终身服药。但仍有10％～15％患者难以控制发作，可以采用外科治疗。
在预防方面：
1.预防癫痫病的发生，应详细地进行家系调查，了解患者双亲同胞和近亲中是否有癫痫发作及其发作特点，对能引起智力低下和癫痫的一些严重遗传性疾病，应进行产前诊断或新生儿期过筛检查，以决定终止妊娠或早期进行治疗。防止分娩意外，新生儿产伤是癫痫发病的重要原因之一，避免产伤对预防癫痫有重要意义。
2.对癫痫患者要及时诊断，及早治疗，治疗越早脑损伤越小，复发越少，预后越好。去除或减轻引起癫痫的原发病如颅内占位性疾病、代谢异常、感染等，对反复发作的病例也有重要意义。
3.癫痫是一种慢性疾病，可迁延数年、甚至数十年之久，因而可对患者身体、精神、婚姻以及社会经济地位等，造成严重的不良影响。患者在家庭关系、学校教育和就业等方面的不幸和挫折、文体活动方面的限制等，可使患者产生耻辱感和悲观心理，从而严重影响患者的身心发育，这就要求社会各界对癫痫患者给予理解和支持。
早诊断早治疗是防治癫痫的有效方法，但目前缺乏癫痫诊断相关的标志物。
鉴于此，特提出本发明。
发明内容
本发明的目的在于提供一种用于辅助癫痫诊断的生物标志物，该生物标志物可以用于辅助癫痫的诊断，为癫痫的诊断提供一种新的思路。
本发明的另一目的在于提供上述生物标志物的应用。
本发明的另一目的在于提供一种辅助癫痫诊断的试剂盒。
本发明的另一目的在于提供一种用于辅助癫痫诊断的基因芯片。
本发明是这样实现的：
第一方面，本发明提供了一种辅助癫痫诊断的生物标志物，其为核酸分子，其相对于正常GPR98基因的编码序列具有突变c.A6812G。
GPR98基因编码的蛋白属于G蛋白偶联受体超家族成员，该蛋白包含一个有7个跨膜蛋白受体的结构域，结合钙离子，在中枢神经系统里面表达。该基因突变与Usher综合征2型和家族性热性惊厥有关。已报到一些选择性剪切转录本。
进一步地，在本发明的一些实施方案中，所述标志物的碱基序列如SEQ ID NO.1所示。
本发明的研究通过对一个癫痫家系患者的成员的GPR98基因的检测测序发现，该家系中患有癫痫的成员的GPR98基因上具有突变c.A6812G，该基因突变导致其对应的GPR98蛋白的第2271位氨基酸由谷氨酰胺(Q)变异为精氨酸(R)，为错义突变。该变异不属于多态性位点，在人群中发生频率极低。在HGMD专业版数据库中未见报道。经家系验证分析，受检人(图1中4号成员)之父(图1中3号成员)该位点无变异，受检人之母(图1中2号成员)该位点杂合变异，受检人之姥爷(图1中1号成员)该位点杂合变异。
因此，含有c.A6812G突变的核酸分子即相对于正常GPR98基因的编码序列具有突变c.A6812G的核酸片段，或者含有突变p.Q2271R的蛋白均可以作为癫痫诊断的生物标志物，从分子水平上为癫痫的诊断提供一种快速、可靠的检测辅助标记物。
第二方面，本发明提供了一种辅助癫痫诊断的生物标志物，其为蛋白或多肽，其相对于正常GPR98蛋白具有突变p.Q2271R。
进一步地，在本发明的一些实施方案中，所述标志物的氨基酸序列如SEQ ID NO.2所示。
另一方面，本发明提供了检测第一方面所述的标志物的引物或探针、或检测权第二方面所述的生物标志物的抗体在制备癫痫辅助诊断试剂盒、癫痫产前诊断试剂盒或新生儿癫痫筛查试剂盒中的应用。
基于本发明的研究成果，本领域技术人员应当很容易将用于检测含有c.A6812G突变的GPR98突变基因的引物或探针，以及用于检测含有p.Q2271R突变的GPR98突变蛋白的抗体用于制备癫痫辅助诊断试剂盒、癫痫产前诊断试剂盒以及新生儿癫痫筛查试剂盒等领域中。
或者将检测含有c.A6812G突变的GPR98突变基因的探针用于制备辅助癫痫诊断的基因芯片中。
进一步，在本发明的一些实施方案中，所述探针相对于正常GPR98基因的编码序列的第6812位的碱基为G。
另一方面，本发明提供了一种辅助癫痫诊断的试剂盒，其含有用于检测第一方面所述的生物标志物的引物或探针；
或者所述试剂盒含有检测第二方面所述的生物标志物的抗体。
另一方面，本发明提供了一种用于辅助癫痫诊断的基因芯片，该基因芯片上含有用于与目标片段特异性结合的探针，所述目标片段性相对于正常GPR98基因的编码序列具有突变c.A6812G。
所述探针与目标片段特异性结合的区域涵盖如下位置：相对于正常GPR98基因的第6812位。
进一步，在本发明的一些实施方案中，所述探针相对于正常GPR98基因编码序列的第6812位的碱基为G。
进一步，在本发明的一些实施方案中，所述目标片段为来自待测主体的GPR98基因的编码序列。
进一步，在本发明的一些实施方案中，所述主体为人。
附图说明
为了更清楚地说明本发明实施例的技术方案，下面将对实施例中所需要使用的附图作简单地介绍，应当理解，以下附图仅示出了本发明的某些实施例，因此不应被看作是对范围的限定，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其他相关的附图。
图1为本发明实施例1中的受检对象癫痫患者家系的遗传家系图谱；
图2-A为本发明实施例1中的遗传家系图谱中的4号成员的GPR98基因测序结果图；
图2-B为本发明实施例1中的遗传家系图谱中的1号成员的GPR98基因测序结果图；
图2-C为本发明实施例1中的遗传家系图谱中的2号成员的GPR98基因测序结果图；
图2-D为本发明实施例1中的遗传家系图谱中的3号成员的GPR98基因测序结果图。
具体实施方式
为使本发明实施例的目的、技术方案和优点更加清楚，下面将对本发明实施例中的技术方案进行清楚、完整地描述。实施例中未注明具体条件者，按照常规条件或制造商建议的条件进行。所用试剂或仪器未注明生产厂商者，均为可以通过市售购买获得的常规产品。
以下结合实施例对本发明的特征和性能作进一步的详细描述。
实施例1
1癫痫患者家系的GPR98基因突变检测
1.1对象：来自重庆市沙坪坝区杨氏癫痫家系先证者及其家系成员，该家系的遗传图谱如图1所示，包括3例癫痫患者(图中1 2 4号成员)，1例正常家庭成员(图中3号成员)；1号、2号、4号：全面强直-阵挛发作；3号：未患病。对所有1-4号家庭成员进行详细体格检查，在签署知情同意书后每人采集血液样本。
1.2测序：
将采集的血液样本送北京迈基诺基因科技股份有限公司测序进行癫痫基因突变筛查及验证，分析临床表型、基因型的关系，结果如下：
将测得的序列与正常GPR98基因标准序列比对(https://www.ncbi.nlm.nih.gov/nuccore/NM_032119.3)，测序结果如图2-A所示，先证者即4号成员GPR98基因有1个杂合突变：c.6812A>G(编码区第6812号核苷酸由腺嘌呤变异为鸟嘌呤)，导致氨基酸改变p.Q2271R(第2271号氨基酸由谷氨酰胺变异为精氨酸)，为错义突变。该变异不属于多态性位点，在人群中发生频率极低。在HGMD专业版数据库中未见报道。经家系验证分析，先证者(4号成员)之父(图1中3号成员)该位点无变异，3号成员的测序结果如图2-D所示，先证者之母(图1中2号成员)该位点杂合变异，2号成员的测序结果如图2-C所示，先证者之姥爷(图1中1号成员)该位点杂合变异，1号成员的测序结果如图2-B所示。图2的A-D中，箭头所示为GPR98基因6812位点的测序结果，1、2、4号成员在该位点的测序结果为均杂合突变(基因型为AG型)，3号成员在该位点的测序结果为无突变(基因型型为AA型)。
c.6812A>G变异信息如下：
综上分析可以看出，GPR98基因6812位点发生突变A>G与癫痫发生相关，该位点可以作为辅助诊断癫痫疾病的靶点，且含有该突变c.A6812G的核酸片段或相应的具有突变p.Q2271R的GPR98蛋白均可以作为辅助诊断癫痫疾病的生物标志物。相应的，用于检测含有具有突变c.A6812G的核酸片段的探针、引物或其他试剂以及检测具有突变p.Q2271R的GPR98蛋白的抗体等均可以用于制备辅助诊断癫痫疾病的制剂盒。
实施例2
本实施例提供了检测GPR98基因第6812位点突变的试剂盒，其包括：用于检测GPR98基因6812位点是否发生突变A6812G的引物，正向引物：TCCACTATAGGATGGTGCTTGG，反向引物：AGCATAGTTTGAACATTCTTGCAG；以及PCR扩增试剂：10×Buffer(含15mM Mg2+)、dNTP(2.5Mm)、高保真DNA聚合酶pfu DNA聚合酶(5U/μl)以及ddH2O。
使用试剂盒的检测GPR98基因第6812位点是否发生突变A>G的方法，主要包括如下步骤：
(1)提取样本DNA，以此为模板，利用上述PCR反应试剂盒进行PCR反应。
(2)多重PCR产物检测：用琼脂糖凝胶对PCR扩增产物进行电泳检测PCR是否成功扩增，扩增片段小461bp。
(3)将PCR产物直接测序，将测序结果与正常GPR98基因序列比对，可判断待测样本的GPR98基因6812位点是否发生突变A>G。
以上所述仅为本发明的优选实施例而已，并不用于限制本发明，对于本领域的技术人员来说，本发明可以有各种更改和变化。凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。
SEQUENCE LISTING
<110> 重庆医科大学附属第一医院
<120> 一种辅助癫痫诊断的标志物及其检测试剂盒
<160> 2
<170> PatentIn version 3.5
<210> 1
<211> 18921
<212> DNA
<213> 人工序列
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atgtcggtgt tcctggggcc agggatgccc tctgcatctt tattagtaaa tcttctttca 60
gctttactca tcctatttgt gtttggagaa acagaaataa gatttactgg acaaactgaa 120
tttgttgtta atgaaacaag tacaacagtt attcgtctta tcattgaaag gataggagag 180
ccagcaaatg ttactgcaat tgtatcgctg tatggagagg acgctggtga cttttttgac 240
acatatgctg cagcttttat acctgccgga gaaacaaaca gaacagtgta catagcagta 300
tgtgatgatg acttaccaga gcctgacgaa acttttattt ttcacttaac attacagaaa 360
ccttcagcaa atgtgaagct tggatggcca aggactgtta ctgtgacaat attatcaaat 420
gacaatgcat ttggaattat ttcatttaat atgcttccct caatcgcagt gagtgagccc 480
aagggcagaa atgagtctat gcctcttact ctcatcaggg aaaagggaac ctatggaatg 540
gtcatggtga cttttgaggt agagggtggc ccaaatcccc ctgatgaaga tttgagtcca 600
gttaaaggaa atatcacctt tccccctggc agagcaacag taatttataa cttgacagta 660
ctcgatgacg aggtaccaga aaatgatgaa atatttttaa ttcaactgaa aagtgtagaa 720
ggaggagctg agattaacac ctctaggaat tccattgaga tcatcattaa gaaaaatgat 780
agtcccgtga gattccttca gagtatttat ttggttcctg aggaagacca catactcata 840
attccagtag ttcgtggaaa ggacaacaat ggaaatctga ttggatctga tgaatatgag 900
gtttcaatca gttatgctgt cacaactggg aattccacag cacatgccca gcaaaatctg 960
gacttcattg atcttcagcc aaacacaact gttgtttttc caccttttat tcatgaatct 1020
cacttgaaat ttcaaatagt tgatgacacc ataccggaga ttgctgaatc gtttcacatt 1080
atgttactaa aagatacctt acagggagat gctgtgctaa taagcccttc tgttgtacaa 1140
gtcaccatta agccaaatga taaaccttat ggagtccttt cattcaacag tgttttgttt 1200
gaaaggacag ttataattga tgaagataga atatcaagat atgaagaaat cacagtggtt 1260
agaaatggag gaacccatgg gaatgtctct gcgaattggg tgttgacacg gaacagcact 1320
gatccctcac cagtaacagc agatatcaga ccgagctctg gagttctcca ttttgcacaa 1380
gggcagatgt tggcaacaat tcctcttact gtggttgatg atgatcttcc agaagaggca 1440
gaagcttatc tacttcaaat tctgcctcat acaatacgag gaggtgcaga agtgagcgag 1500
ccagcggagc ttttgttcta cattcaggat agtgatgatg tctatggcct aataacattt 1560
tttcctatgg aaaaccagaa gattgaaagc agcccaggtg aacgatactt atccttgagt 1620
tttacaagac taggagggac taaaggagat gtgaggttgc tttattctgt actttacatt 1680
cctgctggag ctgtggaccc cttgcaagca aaagaaggca tcttaaatat atcaaggaga 1740
aatgacctca tttttccaga gcaaaaaact caagtcacta caaaattacc aataagaaat 1800
gatgcattcc ttcaaaatgg agctcacttt ctagtacagt tggaaactgt ggagttgtta 1860
aacataattc ctctaatccc acccataagc cctagatttg gggaaatctg caatatttct 1920
ttactggtta ctccagccat tgcaaatgga gaaattggct ttctcagcaa tcttccaatt 1980
attttgcatg aaccagaaga ttttgctgct gaagtggtat acattccctt acatcgggat 2040
ggaactgatg gccaggctac tgtctactgg agtttgaagc cctctggctt taattcaaaa 2100
gcagtgaccc cggatgatat aggccccttt aatggctctg ttttgttttt atctgggcaa 2160
agtgacacaa caatcaacat tactatcaaa ggtgatgaca taccggaaat gaatgaaact 2220
gtaacacttt ctctagacag ggttaacgtg gaaaaccaag tgctgaaatc tggatatact 2280
agccgtgacc taattatttt ggaaaatgat gaccctgggg gagtttttga attttctcct 2340
gcttccagag gaccctatgt tataaaagaa ggagaatctg tagagctcca catcatccga 2400
tcaagggggt cccttgttaa gcagtttcta cactaccgag tagagccaag agatagcaat 2460
gaattctatg gaaacacggg agtactagaa tttaaacctg gagaaaggga gatagtgatc 2520
accttgctag caagattgga tgggatacca gagttggatg aacactactg ggtggtcctc 2580
agcagccacg gagaacggga aagcaagttg ggaagtgcca ccattgtcaa tataacgatt 2640
ctgaaaaatg atgatcctca tggcattata gaatttgttt ctgatggtct aattgtgatg 2700
ataaatgaaa gcaaaggaga tgctatctat agtgctgttt atgatgtagt aagaaatcga 2760
ggcaactttg gtgatgttag tgtatcatgg gtggttagtc cagactttac acaagatgta 2820
tttcctgtac aagggactgt tgtctttgga gatcaggaat tttcaaaaaa tatcaccatt 2880
tactcccttc cagatgagat tccagaagaa atggaagaat ttaccgttat cctactgaat 2940
ggcactggag gagctaaagt gggaaataga acaactgcaa ctctgaggat tagaagaaat 3000
gatgacccca tttattttgc agaacctcgt gtagtgaggg ttcaggaagg tgagactgcc 3060
aactttacag ttctcagaaa tggatctgtt gatgtgactt gcatggtcca gtatgctacc 3120
aaggatggga aggctactgc aagagagaga gatttcattc ctgttgaaaa aggagaaacg 3180
ctcatttttg aggttggaag tagacagcag agcatatcca tatttgttaa tgaagatggt 3240
atcccggaaa cagatgagcc cttttatata atcctcttga attcaacagg tgatacagta 3300
gtatatcaat atggagtagc tacagtaata attgaagcta atgatgaccc aaatggcatt 3360
ttttctctgg agcccataga caaagcagtg gaagaaggaa agactaatgc attttggatt 3420
ttgaggcacc gaggatactt tggtagtgtt tctgtatctt ggcagctctt tcagaatgat 3480
tctgctttgc agcctgggca ggagttctat gaaacttcag gaactgttaa cttcatggat 3540
ggagaagaag caaaaccaat cattctccat gcttttccag ataaaattcc tgaattcaat 3600
gaattttatt tcctaaaact tgtaaacatt tcaggtggat ccccaggtcc tgggggccag 3660
ctagcagaaa ccaacctcca ggtgacagta atggttccat tcaatgatga tccctttgga 3720
gtttttatct tggatccaga gtgtttagag agagaagtgg cagaagatgt cctgtctgaa 3780
gatgatatgt cttatattac caacttcacc attttgaggc agcagggtgt gtttggtgat 3840
gtacaactgg gctgggaaat actgtccagt gagttccctg ctggtttgcc accaatgata 3900
gattttttac tggttggaat tttccccacc accgtgcatt tacaacagca catgcggcgt 3960
caccacagtg gaacggatgc tttgtacttt accggactag agggtgcatt tgggactgtt 4020
aatccaaaat accatccctc caggaataat acaattgcca actttacatt ctcagcttgg 4080
gtaatgccca atgccaatac gaatggattc attatagcga aggatgacgg taatggaagc 4140
atctactacg gggtaaaaat acaaacaaac gaatcccatg tgacactttc ccttcattat 4200
aaaaccttgg gttccaatgc tacatacatt gccaagacaa cagtcatgaa atatttagaa 4260
gaaagtgttt ggcttcatct actaattatc ctggaggatg gtataatcga attctacctg 4320
gatggaaatg caatgcccag gggaatcaag agtctgaaag gagaagccat tactgacggt 4380
cctgggatac tgagaattgg agcagggata aatggcaatg acagatttac aggtctgatg 4440
caggatgtga ggtcctatga gcggaaactg acgcttgaag aaatttatga acttcatgcc 4500
atgcccgcaa aaagtgattt acacccaatt tctggatatc tggagttcag acagggagaa 4560
actaacaaat cattcattat ttctgcaaga gatgacaatg acgaggaagg agaagaatta 4620
ttcattctta aactagtttc tgtatatgga ggagctcgta tttcggaaga aaatactact 4680
gcaagattaa caatacaaaa aagtgacaat gcaaatggct tgtttggttt cacaggagct 4740
tgtataccag agattgcaga ggagggatca accatttctt gtgtggttga gagaaccaga 4800
ggagctctgg attatgtgca tgttttttac accatttcac agattgaaac tgatggcatt 4860
aattaccttg ttgatgactt tgctaatgcc agtggaacta ttacattcct tccttggcag 4920
agatcagagg ttctgaatat atatgttctt gatgatgata ttcctgaact taatgagtat 4980
ttccgtgtga cattggtttc tgcaattcct ggagatggga agctaggctc aactcctacc 5040
agtggtgcaa gcatagatcc tgaaaaggaa acgactgata tcaccatcaa agctagtgat 5100
catccatatg gcttgctgca gttctccaca gggctgcctc ctcagcctaa ggacgcaatg 5160
accctgcctg caagcagcgt tccacatatc actgtggagg aggaagatgg agaaatcagg 5220
ttattggtca tccgtgcaca gggacttctg ggaagggtga ctgcggaatt tagaacagtg 5280
tccttgacag cattcagtcc tgaggattac cagaatgttg ctggcacatt agaatttcaa 5340
ccaggagaaa gatataaata cattttcata aacatcactg ataattctat tcctgaactg 5400
gaaaaatctt ttaaagttga gttgttaaac ttggaaggag gagtagctga actctttagg 5460
gttgatggaa gtggtagtgg tgatggggac atggaattct tccttccaac tattcacaaa 5520
cgtgccagtc taggagtggc ttcccaaatt ctagtgacaa ttgcagcctc tgaccacgct 5580
catggcgtat ttgaatttag ccctgagtca ctctttgtca gtggaactga accagaagat 5640
gggtatagca ctgttacatt aaatgttata agacatcatg gaactctgtc tccagtgact 5700
ttgcattgga acatagactc tgatcctgat ggtgatctcg ccttcacctc tggcaacatc 5760
acatttgaga ttgggcagac gagcgccaat atcactgtgg agatattgcc tgacgaagac 5820
ccagaactgg ataaggcatt ctctgtgtca gtcctcagtg tttccagtgg ttctttggga 5880
gctcatatta atgccacgtt aacagttttg gctagtgatg atccatatgg gatattcatt 5940
ttttctgaga aaaacagacc tgttaaagtt gaggaagcaa cccagaacat cacactatca 6000
ataataaggt tgaaaggcct catgggaaaa gtccttgtct catatgcaac actagatgat 6060
atggaaaaac caccttattt tccacctaat ttagcgagag caactcaagg aagagactat 6120
ataccagctt ctggatttgc tctttttgga gctaatcaga gtgaggcaac aatagctatt 6180
tcaattttgg atgatgatga gccagaaagg tccgaatctg tctttatcga actactcaac 6240
tctactttag tagcgaaagt acagagtcgt tcaattccaa attctccacg tcttgggcct 6300
aaggtagaaa ctattgcgca actaattatc attgccaatg atgatgcatt tggaactctt 6360
cagctctcag caccaattgt ccgagtggca gaaaatcatg ttggacccat tatcaatgtg 6420
actagaacag gaggagcatt tgcagatgtc tctgtgaagt ttaaagctgt gccaataact 6480
gcaatagctg gtgaagatta tagtatagct tcatcagatg tggtcttgct agaaggggaa 6540
accagtaaag ccgtgccaat atatgtcatt aatgatatct atcctgaact ggaagaatct 6600
tttcttgtgc aactgatgaa tgaaacaaca ggaggagcca gactaggggc tttaacagag 6660
gcagtcatta ttattgaggc ctctgatgac ccctatggat tatttggttt tcagattact 6720
aaacttattg tagaggaacc tgagtttaac tcagtgaagg taaacctgcc aataattcga 6780
aattctggga cactcggcaa tgttactgtt cggtgggttg ccaccattaa tggacagctt 6840
gctactggcg acctgcgagt tgtctcaggt aatgtgacct ttgcccctgg ggaaaccatt 6900
caaaccttgt tgttagaggt cctggctgac gacgttccgg agattgaaga ggttatccaa 6960
gtgcaactaa ctgatgcctc tggtggaggt actattgggt tagatcgaat tgcaaatatt 7020
attattcctg ccaatgatga tccttatggt acagtagcct ttgctcagat ggtttatcgt 7080
gttcaagagc ctctggaaag aagttcctgt gctaatataa ctgtcaggcg aagcggaggg 7140
cactttggtc ggctgttgtt gttctacagt acttccgaca ttgatgtagt ggctctggca 7200
atggaggaag gtcaagattt actgtcctac tatgaatctc caattcaagg ggtgcctgac 7260
ccactttgga gaacttggat gaatgtctct gccgtggggg agcccctgta tacctgtgcc 7320
actttgtgcc ttaaggaaca agcttgctca gcgttttcat ttttcagtgc ttctgagggt 7380
ccccagtgtt tctggatgac atcatggatc agcccagctg tcaacaattc agacttctgg 7440
acctacagga aaaacatgac cagggtagca tctcttttta gtggtcaggc tgtggctggg 7500
agtgactatg agcctgtgac aaggcaatgg gccataatgc aggaaggtga tgaattcgca 7560
aatctcacag tgtctattct tcctgatgat ttcccagaga tggatgagag ttttctaatt 7620
tctctccttg aagttcacct catgaacatt tcagccagtt tgaaaaatca gccaaccata 7680
ggacagccaa atatttctac agttgtcata gcactaaatg gtgatgcctt tggagtgttt 7740
gtgatctaca atattagtcc caatacttcc gaagatggct tatttgttga agttcaggag 7800
cagccccaaa ccttggtgga gctgatgata cacaggacag ggggcagctt aggtcaagtg 7860
gcagtcgaat ggcgtgttgt tggtggaaca gctactgaag gtttagattt tataggtgct 7920
ggagagattc tgacctttgc tgaaggtgaa accaaaaaga cagtcatttt aaccatcttg 7980
gatgactctg aaccagagga tgacgaaagt atcatagtta gtttggtgta cactgaaggt 8040
ggaagtagaa ttttgccaag ctccgacact gttagagtga acattttggc caatgacaat 8100
gtggcaggaa ttgttagctt tcagacagct tccagatctg tcataggtca tgaaggagaa 8160
attttacaat tccatgtgat aagaactttc cctggtcgag gaaatgttac tgttaactgg 8220
aaaattattg ggcaaaatct agaactcaat tttgctaact ttagcggaca acttttcttt 8280
cctgaggggt cgttgaatac aacattgttt gtgcatttgt tggatgacaa cattcctgag 8340
gagaaagaag tataccaagt cattctgtat gatgtcagga cacaaggagt tccaccagcc 8400
ggaatcgccc tgcttgatgc tcaaggatat gcagctgtcc tcacagtaga agccagtgat 8460
gaaccacatg gagttttaaa ttttgctctt tcatcaagat ttgtgttact acaagaggct 8520
aacataacaa ttcagctttt catcaacaga gaatttggat ctctaggagc tatcaatgtc 8580
acatatacca cggttcctgg aatgctgagt ctgaagaacc aaacagtagg aaacctagca 8640
gagccagaag ttgattttgt ccctatcatt ggctttctga ttttagaaga aggggaaaca 8700
gcagcagcca tcaacattac cattcttgag gatgatgtac cagagctaga agaatatttc 8760
ctggtgaatt taacttacgt tggacttacc atggctgctt caacttcatt tcctcccaga 8820
ctagattcag aaggtttgac tgcacaagtt attattgatg ccaatgatgg ggcccgaggt 8880
gtaattgaat ggcaacaaag caggtttgaa gtaaatgaaa cccatggaag tttaacattg 8940
gtagcccaga ggagcagaga acctcttggc catgtttcct tatttgtgta tgctcagaat 9000
ttggaagcac aagtggggct ggattatatc ttcaccccaa tgattcttca ttttgctgat 9060
ggagaaaggt ataaaaatgt caatatcatg attcttgatg atgacattcc agaaggagat 9120
gaaaaatttc agctgatttt aacaaatcct tctcctggac tagagctagg gaaaaataca 9180
atagccttaa ttattgtcct tgctaatgat gacggccctg gagttctatc atttaacaac 9240
agtgagcact ttttcctaag agagccaaca gctctctacg tccaggagag tgttgcagta 9300
ttgtacattg ttcgggaacc tgcacaagga ttgtttggaa cagtgacagt tcagttcatt 9360
gtgacagaag tgaattcctc aaatgaatct aaagatctga ctccttccaa aggctatatt 9420
gttttagaag aaggtgttcg attcaaggcc ctacaaatat ctgccatatt agacacggaa 9480
ccagaaatgg atgagtattt tgtttgcacc ttgtttaatc caactggagg tgctagacta 9540
ggggtgcatg ttcaaaccct gataacagtt ttgcaaaacc aggccccttt ggggctattc 9600
agtatctctg cagttgaaaa tagagccacc tccatagaca tcgaagaagc caataggacc 9660
gtgtatttaa atgtatctcg aactaatggc attgatttgg ctgtgagtgt gcagtgggag 9720
acagtatctg aaacagcctt tggcatgagg ggaatggatg ttgtgttttc cgtatttcaa 9780
agttttttgg atgaatcagc ttctggctgg tgtttcttta ctttggaaaa tttaatatat 9840
ggtataatgt taagaaaatc atctgttact gtttaccgat ggcaggggat ttttattcca 9900
gttgaggatt taaatataga aaatcctaaa acttgtgagg cctttaatat tggtttttct 9960
ccctactttg tgattactca tgaagaaaga aatgaagaaa agccttctct taacagtgtg 10020
tttacattca catctggatt taaattattc ctggtacaaa caatcattat tctggaaagt 10080
tctcaagtaa gatattttac ttcagacagc caagattatt taatcattgc aagtcaaaga 10140
gatgattccg aattaactca ggtcttcagg tggaatggag gaagcttcgt gttgcatcaa 10200
aaactccctg tccgaggtgt gctgaccgtg gccttgttca acaagggagg ctctgtgttc 10260
ttagccattt cccaggctaa tgccaggcta aactcccttt tattcagatg gtctggcagt 10320
gggtttatta actttcaaga ggtgcctgtc agtgggacaa cagaagttga ggctttgtct 10380
tcagccaatg atatttacct aatatttgcc gaaaatgtct ttctaggaga tcagaattca 10440
attgatattt tcatctggga gatgggacag tcttccttca ggtattttca gtctgtagat 10500
tttgctgctg ttaacagaat ccactccttc acaccagcct caggaatagc ccacatactt 10560
cttattggcc aagatatgtc tgctctttac tgctggaatt cggagcgtaa tcaattctct 10620
tttgttctgg aagtaccttc tgcttatgat gtggcttctg ttacagtaaa gtcccttaat 10680
tcaagcaaga atttaatagc tctagtggga gctcattcac atatatatga gctagcctac 10740
atttccagcc attctgactt tattcctagt tcaggtgaac tgatatttga acctggtgag 10800
agagaagcta caatagcagt aaatatcctt gatgatacag ttccagaaaa agaagaatcc 10860
ttcaaagttc aacttaaaaa tcccaaagga ggagcagaga ttggcattaa tgattctgta 10920
acaataacca ttctgtctaa tgatgatgcc tatggaattg ttgcatttgc tcagaattca 10980
ttatataagc aagtggaaga aatggagcaa gatagcctag taaccttgaa cgttgaacgc 11040
ttaaaaggaa catatggccg tataaccata gcatgggaag ctgatggaag tattagtgat 11100
atatttccta cctcaggagt gattttattt actgaaggcc aggtactgtc aacaatcact 11160
ctaactattc ttgctgataa tataccagag ttatcagagg ttgtgattgt aaccctcacc 11220
cgtatcacca cagaaggggt tgaggactca tacaaaggtg ctactattga tcaggacaga 11280
agcaagtctg ttataacaac tttgcccaat gactcacctt ttggcttggt gggctggcgt 11340
gctgcgtctg tcttcattag agtagcagag cctaaagaaa acaccaccac tcttcagtta 11400
caaatagctc gagataaagg actacttggg gatattgcca ttcacttgag agctcaaccc 11460
aatttcttac tgcatgtcga taatcaagct actgagaatg aagattatgt attgcaagaa 11520
acaataataa taatgaaaga aaacataaaa gaagctcatg ccgaagtttc cattttgccg 11580
gatgaccttc ctgaattgga ggaaggattt attgtcacta tcactgaggt gaacctggtg 11640
aactctgact tctctacagg acagccaagt gtgcggaggc ccggaatgga aatagctgag 11700
ataatgatag aagaaaatga cgatcccaga ggaattttta tgtttcatgt tactagaggc 11760
gctggggaag ttattactgc ctatgaggtg cctccaccct tgaacgttct tcaagttcct 11820
gtagtccggc tggctggaag ctttggggca gtaaatgttt attggaaagc atcaccagac 11880
agtgctggcc tggaagactt taaaccatct catgggattc ttgaatttgc agataaacag 11940
gttactgcaa tgatagaaat caccataatt gatgatgctg aatttgaatt gacagagacg 12000
ttcaatattt ccttgatcag tgttgctgga ggtggcagac ttggtgatga tgttgtggta 12060
actgttgtta ttccacaaaa tgattctcca tttggagtat ttggatttga agaaaagact 12120
gtaatgattg atgaatccct ttcatccgat gaccctgatt catatgtgac attgacggtt 12180
gtccggtccc caggaggaaa aggaaccgtc cgacttgagt ggaccataga tgagaaggct 12240
aaacataacc ttagtccttt gaatgggacc cttcattttg atgagactga gtcccagaag 12300
accattgtgt tgcacacact tcaagacaca gtgttggagg aggacaggcg tttcaccatt 12360
cagctgatat caattgatga ggtagaaata tctccagtaa aaggtagtgc atcaataatt 12420
attcggggtg ataagcgagc atcaggagaa gttgggatag ctccgtcatc taggcacatc 12480
ctcattgggg aaccctcagc aaaatataat ggtaccgcta ttatcagcct tgttcgaggc 12540
ccagggattt tgggggaggt cacagtgttc tggaggatat tccctccttc cgtgggggaa 12600
tttgctgaaa catcaggaaa actgacaatg cgagacgaac agtctgcagt cattgtagta 12660
atacaggctt tgaacgatga cattcccgag gaaaaaagct tctatgagtt tcagctcact 12720
gcagtcagtg agggaggagt tctgagtgaa tccagcagca ctgccaacat cacggtggtg 12780
gccagcgact ctccctatgg ccgatttgcc ttttcacatg agcaacttcg agtgtcagaa 12840
gcacagaggg ttaacatcac aatcatccgt tccagtggag attttggcca tgtgcgactc 12900
tggtacaaga cgatgagcgg gacagcggaa gcaggcttgg attttgttcc tgcagcaggg 12960
gagctcctct ttgaagcagg ggagatgagg aaaagtctgc atgttgaaat ccttgatgat 13020
gactatcctg aaggcccaga ggaattttct ctaacaatta caaaggtgga actccaggga 13080
agagggtatg attttaccat tcaagaaaat ggacttcaga tagatcaacc tcctgaaata 13140
ggaaacatct ccattgttcg catcataata atgaaaaatg ataacgcaga aggcatcatt 13200
gaatttgacc caaagtatac tgccttcgaa gtggaggaag atgttgggct gatcatgatc 13260
ccagtggtga ggctacatgg aacttatggc tatgtgacag ctgatttcat ctctcagagc 13320
tcctctgcca gtcccggagg tgttgattac attttgcatg gcagtacagt cacctttcag 13380
catgggcaaa acttaagttt tataaatatc tccatcattg atgacaatga aagtgaattt 13440
gaggagccca ttgaaattct actcactgga gctactggag gagcggtcct tgggcgccac 13500
ctagtgagca gaatcataat agctaagagt gactctccct ttggagttat aaggtttctc 13560
aatcaaagca aaatttctat tgctaatccc aattccacaa tgattttatc actggtgctg 13620
gagcggactg gaggactctt gggagagatt caggtgaact gggagacagt aggacccaac 13680
tctcaagaag ccttactgcc acagaataga gacattgcag acccagtgag cgggttgttc 13740
tattttggag aaggagaagg aggagtgaga accataattc tgacaatcta tcctcatgaa 13800
gaaattgaag ttgaagagac attcattatt aaacttcatc ttgtgaaagg agaagctaaa 13860
ttagactcca gagctaaaga tgttacatta accatacaag agtttggtga cccaaatgga 13920
gttgttcagt ttgctcctga aactttgtct aagaagactt attcagagcc tctggctctg 13980
gaagggcccc tgctcattac cttctttgtc agaagagtca agggcacctt tggagagatt 14040
atggtttact gggaattaag tagtgagttt gacattactg aagactttct ttccaccagt 14100
ggatttttca ccattgctga tggagagagt gaagctagct ttgatgttca tttgctacca 14160
gatgaggtac ctgagataga ggaagattat gtgatccagc ttgtttctgt agagggagga 14220
gccgaactgg atctggagaa gagtatcaca tggttctctg tttatgcaaa tgatgaccca 14280
catggagtat ttgccctgta ttcggatcgc cagtcaatac ttattgggca gaaccttatt 14340
agatccatcc aaattaacat aacccggctt gctggaacat ttggagatgt ggctgttggg 14400
cttcgaatat catcggatca taaagaacag ccgattgtta ccgaaaatgc agagaggcag 14460
ctggtggtca aagatggtgc cacatataaa gtggacgtgg tgccaataaa gaatcaggtc 14520
ttcctatcac tgggctctaa tttcactttg caactggtga ctgtgatgct tgtcggtgga 14580
cgtttctatg gaatgccaac aattcttcag gaagcaaaat ctgctgtcct tccagtctct 14640
gagaaagctg ccaattctca ggtcggattt gaatccactg cttttcaact catgaacatc 14700
actgctggca caagccacgt tatgatttct aggagaggca catatggagc tctctcggtt 14760
gcctggacca ctggatatgc tcctgggtta gaaattcctg aattcattgt tgttggcaac 14820
atgaccccaa cactggggag cctttcattt tcccacggtg aacaaaggaa aggagttttc 14880
ctgtggacgt ttcctagccc tggttggcca gaggcctttg ttcttcacct atcaggagtg 14940
cagagcagtg ctcctggcgg agctcaactc cgatcaggtt tcattgttgc tgaaattgaa 15000
ccaatgggcg tcttccaatt ttccactagc tcaagaaata tcatagtgtc agaagataca 15060
cagatgatca gattacatgt acaaagacta tttgggttcc acagcgatct tattaaagtt 15120
tcttatcaga ccactgcagg aagcgccaag ccactggaag attttgagcc tgttcagaat 15180
ggggaactgt tttttcaaaa attccaaact gaggttgatt ttgaaataac cattattaat 15240
gatcagcttt ctgagataga agaatttttt tacattaacc ttacttcagt agaaattagg 15300
ggattacaaa agtttgatgt taattggagc ccacgcctga atctagattt cagtgttgca 15360
gtgattacaa tattggataa tgatgacctg gcaggaatgg atatttcctt ccccgagaca 15420
actgtggctg tagcagttga cacaactctc attcctgtag aaactgaatc caccacatac 15480
ctcagcacaa gcaagacgac taccattctg cagccaacca acgtggttgc cattgttact 15540
gaggcaactg gtgtatctgc catccctgag aaacttgtca cccttcatgg cacacctgct 15600
gtgtctgaaa agcctgatgt ggccactgta actgccaatg tttccattca tggaacattc 15660
agccttgggc catccattgt ttatattgaa gaggagatga agaatggcac attcaacact 15720
gcagaagttc ttatccgaag aactggtggg tttactggca atgtcagcat aacagttaaa 15780
actttcggtg aaagatgtgc tcagatggaa ccaaatgcat tgccctttcg tggtatctat 15840
gggatttcca acctaacatg ggcagttgaa gaagaagact ttgaagaaca aactcttacc 15900
cttatattcc tagatggaga aagagaacgt aaagtatcag ttcaaatttt ggatgatgat 15960
gagcctgagg ggcaggaatt cttctacgtg tttctcacaa accctcaagg gggagcacag 16020
attgtggagg agaaggatga tactggattt gcagcttttg ccatggttat tattacaggg 16080
agtgaccttc acaatggcat cataggattc agtgaggagt cccagagtgg actagaactc 16140
agggaaggag ctgttatgag aagattgcac cttattgtca caagacagcc aaacagggcc 16200
tttgaagatg tcaaggtctt ttggcgagtc acacttaaca aaacagtcgt cgtgctccag 16260
aaggatgggg taaacctggt ggaggaactt cagtctgtgt cagggaccac aacctgtaca 16320
atgggtcaaa caaaatgctt tatcagcatt gaactcaaac cagaaaaggt accacaggtt 16380
gaagtgtatt tttttgtgga actatatgaa gctactgctg gagcagcaat aaacaacagt 16440
gccagattcg cacagattaa aatcttagaa agtgatgaat ctcaaagcct tgtgtatttt 16500
tctgtgggtt ctcggctggc agtggctcac aagaaggcca ctttaatcag tctgcaggtg 16560
gccagagatt ctgggacagg actaatgatg tctgttaact ttagtaccca ggagttgagg 16620
agtgctgaaa caattggtcg taccatcata tctccagcta tttctggaaa ggattttgtg 16680
ataactgaag gcacattggt ctttgaacct ggccagagaa gcactgtatt ggatgtcatc 16740
ctaacgccag agacaggatc tttaaattca tttcctaaac gcttccagat tgtccttttt 16800
gacccaaaag gtggtgccag aattgataaa gtgtatggga ctgccaacat cactcttgtc 16860
tcagatgcag attcgcaggc catttggggg cttgcagatc agctacatca gcctgtgaat 16920
gatgatattc tcaacagagt gctccatacc atcagcatga aagtggccac agaaaacaca 16980
gatgaacaac tcagtgccat gatgcattta atagaaaaga taactactga aggaaaaatt 17040
caagctttca gtgttgccag ccgaactctt ttctatgaga ttctttgttc tcttattaac 17100
ccaaagcgca aggacactag gggattcagt cactttgctg aagtgactga gaattttgcc 17160
ttttctctgc tgactaatgt tacttgcggc tctcctggtg aaaaaagcaa aaccatcctt 17220
gatagttgcc catatttgtc aatattggct cttcactggt atcctcagca aatcaatgga 17280
cacaagtttg aaggaaagga aggagattac attcgaattc cagagaggct actggatgtc 17340
caggatgcag aaataatggc tgggaaaagt acatgtaaat tagtccagtt tacagagtat 17400
agcagccaac agtggtttat aagtggaaac aatcttccta ccctaaaaaa taaggtatta 17460
tctttgagtg tgaaaggtca gagttcacaa ctcctgacta atgacaatga ggttctctac 17520
aggatttatg ctgctgagcc tagaattatt cctcagacat ctctgtgtct cctttggaat 17580
caggctgctg caagctggtt gtctgacagt cagttttgca aagtggttga ggaaactgca 17640
gactatgtgg aatgtgcctg ttcacacatg tctgtgtatg ctgtctatgc tcggactgac 17700
aacttgtctt catacaatga agccttcttc acttctggat ttatatgtat ctcaggtctt 17760
tgcttggctg ttctttccca tatcttctgt gccaggtact ccatgtttgc agctaaactt 17820
ctgactcaca tgatggcagc cagcttaggt acacagattc tgtttctggc gtctgcatac 17880
gcaagtcccc aactcgctga ggagagctgt tcagctatgg ctgctgtcac acattacctg 17940
tatctttgcc agtttagctg gatgctcatt cagtctgtga atttctggta cgtgctggtg 18000
atgaatgatg agcacacaga gaggcgatat ctgctgtttt tccttctgag ttggggacta 18060
ccagcttttg tggtgattct cctcatagtt attttgaaag gaatctatca tcagagcatg 18120
tcacagatct atggactcat tcatggtgac ctgtgtttta ttccaaacgt ctatgctgct 18180
ttgttcactg cagctcttgt tcctttgacg tgcctcgtgg tggtgttcgt ggtgttcatc 18240
catgcctacc aggtgaagcc acagtggaaa gcatatgatg atgtcttcag aggaaggaca 18300
aatgctgcag aaattccact gattttatat ctctttgctc tgatttccgt gacatggctt 18360
tggggaggac tacacatggc ctacagacac ttctggatgt tggttctctt tgtcattttc 18420
aacagtctgc agggacttta tgttttcatg gtttatttca ttttacacaa ccaaatgtgt 18480
tgccctatga aggccagtta cactgtggaa atgaatgggc atcctggacc cagcacagcc 18540
tttttcacgc ccgggagtgg aatgcctcct gctggagggg aaatcagcaa gtccacccag 18600
aatctcatcg gtgctatgga ggaggtgcca cctgactggg agagagcatc cttccaacag 18660
ggcagtcagg ccagccctga tttaaagcca agtccacaaa atggagccac gttcccgtcc 18720
tctggaggat atggccaggg gtcactgata gccgatgagg agtcccagga gtttgatgat 18780
ttaatatttg cattaaaaac tggtgctggt ctcagtgtca gtgataatga atctggtcaa 18840
ggcagccagg aggggggcac cttgactgac tcccagatcg tggagctcag gaggataccc 18900
atcgccgaca ctcacctgta g 18921
<210> 2
<211> 6306
<212> PRT
<213> 人工序列
<400> 2
Met Ser Val Phe Leu Gly Pro Gly Met Pro Ser Ala Ser Leu Leu Val
1 5 10 15
Asn Leu Leu Ser Ala Leu Leu Ile Leu Phe Val Phe Gly Glu Thr Glu
20 25 30
Ile Arg Phe Thr Gly Gln Thr Glu Phe Val Val Asn Glu Thr Ser Thr
35 40 45
Thr Val Ile Arg Leu Ile Ile Glu Arg Ile Gly Glu Pro Ala Asn Val
50 55 60
Thr Ala Ile Val Ser Leu Tyr Gly Glu Asp Ala Gly Asp Phe Phe Asp
65 70 75 80
Thr Tyr Ala Ala Ala Phe Ile Pro Ala Gly Glu Thr Asn Arg Thr Val
85 90 95
Tyr Ile Ala Val Cys Asp Asp Asp Leu Pro Glu Pro Asp Glu Thr Phe
100 105 110
Ile Phe His Leu Thr Leu Gln Lys Pro Ser Ala Asn Val Lys Leu Gly
115 120 125
Trp Pro Arg Thr Val Thr Val Thr Ile Leu Ser Asn Asp Asn Ala Phe
130 135 140
Gly Ile Ile Ser Phe Asn Met Leu Pro Ser Ile Ala Val Ser Glu Pro
145 150 155 160
Lys Gly Arg Asn Glu Ser Met Pro Leu Thr Leu Ile Arg Glu Lys Gly
165 170 175
Thr Tyr Gly Met Val Met Val Thr Phe Glu Val Glu Gly Gly Pro Asn
180 185 190
Pro Pro Asp Glu Asp Leu Ser Pro Val Lys Gly Asn Ile Thr Phe Pro
195 200 205
Pro Gly Arg Ala Thr Val Ile Tyr Asn Leu Thr Val Leu Asp Asp Glu
210 215 220
Val Pro Glu Asn Asp Glu Ile Phe Leu Ile Gln Leu Lys Ser Val Glu
225 230 235 240
Gly Gly Ala Glu Ile Asn Thr Ser Arg Asn Ser Ile Glu Ile Ile Ile
245 250 255
Lys Lys Asn Asp Ser Pro Val Arg Phe Leu Gln Ser Ile Tyr Leu Val
260 265 270
Pro Glu Glu Asp His Ile Leu Ile Ile Pro Val Val Arg Gly Lys Asp
275 280 285
Asn Asn Gly Asn Leu Ile Gly Ser Asp Glu Tyr Glu Val Ser Ile Ser
290 295 300
Tyr Ala Val Thr Thr Gly Asn Ser Thr Ala His Ala Gln Gln Asn Leu
305 310 315 320
Asp Phe Ile Asp Leu Gln Pro Asn Thr Thr Val Val Phe Pro Pro Phe
325 330 335
Ile His Glu Ser His Leu Lys Phe Gln Ile Val Asp Asp Thr Ile Pro
340 345 350
Glu Ile Ala Glu Ser Phe His Ile Met Leu Leu Lys Asp Thr Leu Gln
355 360 365
Gly Asp Ala Val Leu Ile Ser Pro Ser Val Val Gln Val Thr Ile Lys
370 375 380
Pro Asn Asp Lys Pro Tyr Gly Val Leu Ser Phe Asn Ser Val Leu Phe
385 390 395 400
Glu Arg Thr Val Ile Ile Asp Glu Asp Arg Ile Ser Arg Tyr Glu Glu
405 410 415
Ile Thr Val Val Arg Asn Gly Gly Thr His Gly Asn Val Ser Ala Asn
420 425 430
Trp Val Leu Thr Arg Asn Ser Thr Asp Pro Ser Pro Val Thr Ala Asp
435 440 445
Ile Arg Pro Ser Ser Gly Val Leu His Phe Ala Gln Gly Gln Met Leu
450 455 460
Ala Thr Ile Pro Leu Thr Val Val Asp Asp Asp Leu Pro Glu Glu Ala
465 470 475 480
Glu Ala Tyr Leu Leu Gln Ile Leu Pro His Thr Ile Arg Gly Gly Ala
485 490 495
Glu Val Ser Glu Pro Ala Glu Leu Leu Phe Tyr Ile Gln Asp Ser Asp
500 505 510
Asp Val Tyr Gly Leu Ile Thr Phe Phe Pro Met Glu Asn Gln Lys Ile
515 520 525
Glu Ser Ser Pro Gly Glu Arg Tyr Leu Ser Leu Ser Phe Thr Arg Leu
530 535 540
Gly Gly Thr Lys Gly Asp Val Arg Leu Leu Tyr Ser Val Leu Tyr Ile
545 550 555 560
Pro Ala Gly Ala Val Asp Pro Leu Gln Ala Lys Glu Gly Ile Leu Asn
565 570 575
Ile Ser Arg Arg Asn Asp Leu Ile Phe Pro Glu Gln Lys Thr Gln Val
580 585 590
Thr Thr Lys Leu Pro Ile Arg Asn Asp Ala Phe Leu Gln Asn Gly Ala
595 600 605
His Phe Leu Val Gln Leu Glu Thr Val Glu Leu Leu Asn Ile Ile Pro
610 615 620
Leu Ile Pro Pro Ile Ser Pro Arg Phe Gly Glu Ile Cys Asn Ile Ser
625 630 635 640
Leu Leu Val Thr Pro Ala Ile Ala Asn Gly Glu Ile Gly Phe Leu Ser
645 650 655
Asn Leu Pro Ile Ile Leu His Glu Pro Glu Asp Phe Ala Ala Glu Val
660 665 670
Val Tyr Ile Pro Leu His Arg Asp Gly Thr Asp Gly Gln Ala Thr Val
675 680 685
Tyr Trp Ser Leu Lys Pro Ser Gly Phe Asn Ser Lys Ala Val Thr Pro
690 695 700
Asp Asp Ile Gly Pro Phe Asn Gly Ser Val Leu Phe Leu Ser Gly Gln
705 710 715 720
Ser Asp Thr Thr Ile Asn Ile Thr Ile Lys Gly Asp Asp Ile Pro Glu
725 730 735
Met Asn Glu Thr Val Thr Leu Ser Leu Asp Arg Val Asn Val Glu Asn
740 745 750
Gln Val Leu Lys Ser Gly Tyr Thr Ser Arg Asp Leu Ile Ile Leu Glu
755 760 765
Asn Asp Asp Pro Gly Gly Val Phe Glu Phe Ser Pro Ala Ser Arg Gly
770 775 780
Pro Tyr Val Ile Lys Glu Gly Glu Ser Val Glu Leu His Ile Ile Arg
785 790 795 800
Ser Arg Gly Ser Leu Val Lys Gln Phe Leu His Tyr Arg Val Glu Pro
805 810 815
Arg Asp Ser Asn Glu Phe Tyr Gly Asn Thr Gly Val Leu Glu Phe Lys
820 825 830
Pro Gly Glu Arg Glu Ile Val Ile Thr Leu Leu Ala Arg Leu Asp Gly
835 840 845
Ile Pro Glu Leu Asp Glu His Tyr Trp Val Val Leu Ser Ser His Gly
850 855 860
Glu Arg Glu Ser Lys Leu Gly Ser Ala Thr Ile Val Asn Ile Thr Ile
865 870 875 880
Leu Lys Asn Asp Asp Pro His Gly Ile Ile Glu Phe Val Ser Asp Gly
885 890 895
Leu Ile Val Met Ile Asn Glu Ser Lys Gly Asp Ala Ile Tyr Ser Ala
900 905 910
Val Tyr Asp Val Val Arg Asn Arg Gly Asn Phe Gly Asp Val Ser Val
915 920 925
Ser Trp Val Val Ser Pro Asp Phe Thr Gln Asp Val Phe Pro Val Gln
930 935 940
Gly Thr Val Val Phe Gly Asp Gln Glu Phe Ser Lys Asn Ile Thr Ile
945 950 955 960
Tyr Ser Leu Pro Asp Glu Ile Pro Glu Glu Met Glu Glu Phe Thr Val
965 970 975
Ile Leu Leu Asn Gly Thr Gly Gly Ala Lys Val Gly Asn Arg Thr Thr
980 985 990
Ala Thr Leu Arg Ile Arg Arg Asn Asp Asp Pro Ile Tyr Phe Ala Glu
995 1000 1005
Pro Arg Val Val Arg Val Gln Glu Gly Glu Thr Ala Asn Phe Thr
1010 1015 1020
Val Leu Arg Asn Gly Ser Val Asp Val Thr Cys Met Val Gln Tyr
1025 1030 1035
Ala Thr Lys Asp Gly Lys Ala Thr Ala Arg Glu Arg Asp Phe Ile
1040 1045 1050
Pro Val Glu Lys Gly Glu Thr Leu Ile Phe Glu Val Gly Ser Arg
1055 1060 1065
Gln Gln Ser Ile Ser Ile Phe Val Asn Glu Asp Gly Ile Pro Glu
1070 1075 1080
Thr Asp Glu Pro Phe Tyr Ile Ile Leu Leu Asn Ser Thr Gly Asp
1085 1090 1095
Thr Val Val Tyr Gln Tyr Gly Val Ala Thr Val Ile Ile Glu Ala
1100 1105 1110
Asn Asp Asp Pro Asn Gly Ile Phe Ser Leu Glu Pro Ile Asp Lys
1115 1120 1125
Ala Val Glu Glu Gly Lys Thr Asn Ala Phe Trp Ile Leu Arg His
1130 1135 1140
Arg Gly Tyr Phe Gly Ser Val Ser Val Ser Trp Gln Leu Phe Gln
1145 1150 1155
Asn Asp Ser Ala Leu Gln Pro Gly Gln Glu Phe Tyr Glu Thr Ser
1160 1165 1170
Gly Thr Val Asn Phe Met Asp Gly Glu Glu Ala Lys Pro Ile Ile
1175 1180 1185
Leu His Ala Phe Pro Asp Lys Ile Pro Glu Phe Asn Glu Phe Tyr
1190 1195 1200
Phe Leu Lys Leu Val Asn Ile Ser Gly Gly Ser Pro Gly Pro Gly
1205 1210 1215
Gly Gln Leu Ala Glu Thr Asn Leu Gln Val Thr Val Met Val Pro
1220 1225 1230
Phe Asn Asp Asp Pro Phe Gly Val Phe Ile Leu Asp Pro Glu Cys
1235 1240 1245
Leu Glu Arg Glu Val Ala Glu Asp Val Leu Ser Glu Asp Asp Met
1250 1255 1260
Ser Tyr Ile Thr Asn Phe Thr Ile Leu Arg Gln Gln Gly Val Phe
1265 1270 1275
Gly Asp Val Gln Leu Gly Trp Glu Ile Leu Ser Ser Glu Phe Pro
1280 1285 1290
Ala Gly Leu Pro Pro Met Ile Asp Phe Leu Leu Val Gly Ile Phe
1295 1300 1305
Pro Thr Thr Val His Leu Gln Gln His Met Arg Arg His His Ser
1310 1315 1320
Gly Thr Asp Ala Leu Tyr Phe Thr Gly Leu Glu Gly Ala Phe Gly
1325 1330 1335
Thr Val Asn Pro Lys Tyr His Pro Ser Arg Asn Asn Thr Ile Ala
1340 1345 1350
Asn Phe Thr Phe Ser Ala Trp Val Met Pro Asn Ala Asn Thr Asn
1355 1360 1365
Gly Phe Ile Ile Ala Lys Asp Asp Gly Asn Gly Ser Ile Tyr Tyr
1370 1375 1380
Gly Val Lys Ile Gln Thr Asn Glu Ser His Val Thr Leu Ser Leu
1385 1390 1395
His Tyr Lys Thr Leu Gly Ser Asn Ala Thr Tyr Ile Ala Lys Thr
1400 1405 1410
Thr Val Met Lys Tyr Leu Glu Glu Ser Val Trp Leu His Leu Leu
1415 1420 1425
Ile Ile Leu Glu Asp Gly Ile Ile Glu Phe Tyr Leu Asp Gly Asn
1430 1435 1440
Ala Met Pro Arg Gly Ile Lys Ser Leu Lys Gly Glu Ala Ile Thr
1445 1450 1455
Asp Gly Pro Gly Ile Leu Arg Ile Gly Ala Gly Ile Asn Gly Asn
1460 1465 1470
Asp Arg Phe Thr Gly Leu Met Gln Asp Val Arg Ser Tyr Glu Arg
1475 1480 1485
Lys Leu Thr Leu Glu Glu Ile Tyr Glu Leu His Ala Met Pro Ala
1490 1495 1500
Lys Ser Asp Leu His Pro Ile Ser Gly Tyr Leu Glu Phe Arg Gln
1505 1510 1515
Gly Glu Thr Asn Lys Ser Phe Ile Ile Ser Ala Arg Asp Asp Asn
1520 1525 1530
Asp Glu Glu Gly Glu Glu Leu Phe Ile Leu Lys Leu Val Ser Val
1535 1540 1545
Tyr Gly Gly Ala Arg Ile Ser Glu Glu Asn Thr Thr Ala Arg Leu
1550 1555 1560
Thr Ile Gln Lys Ser Asp Asn Ala Asn Gly Leu Phe Gly Phe Thr
1565 1570 1575
Gly Ala Cys Ile Pro Glu Ile Ala Glu Glu Gly Ser Thr Ile Ser
1580 1585 1590
Cys Val Val Glu Arg Thr Arg Gly Ala Leu Asp Tyr Val His Val
1595 1600 1605
Phe Tyr Thr Ile Ser Gln Ile Glu Thr Asp Gly Ile Asn Tyr Leu
1610 1615 1620
Val Asp Asp Phe Ala Asn Ala Ser Gly Thr Ile Thr Phe Leu Pro
1625 1630 1635
Trp Gln Arg Ser Glu Val Leu Asn Ile Tyr Val Leu Asp Asp Asp
1640 1645 1650
Ile Pro Glu Leu Asn Glu Tyr Phe Arg Val Thr Leu Val Ser Ala
1655 1660 1665
Ile Pro Gly Asp Gly Lys Leu Gly Ser Thr Pro Thr Ser Gly Ala
1670 1675 1680
Ser Ile Asp Pro Glu Lys Glu Thr Thr Asp Ile Thr Ile Lys Ala
1685 1690 1695
Ser Asp His Pro Tyr Gly Leu Leu Gln Phe Ser Thr Gly Leu Pro
1700 1705 1710
Pro Gln Pro Lys Asp Ala Met Thr Leu Pro Ala Ser Ser Val Pro
1715 1720 1725
His Ile Thr Val Glu Glu Glu Asp Gly Glu Ile Arg Leu Leu Val
1730 1735 1740
Ile Arg Ala Gln Gly Leu Leu Gly Arg Val Thr Ala Glu Phe Arg
1745 1750 1755
Thr Val Ser Leu Thr Ala Phe Ser Pro Glu Asp Tyr Gln Asn Val
1760 1765 1770
Ala Gly Thr Leu Glu Phe Gln Pro Gly Glu Arg Tyr Lys Tyr Ile
1775 1780 1785
Phe Ile Asn Ile Thr Asp Asn Ser Ile Pro Glu Leu Glu Lys Ser
1790 1795 1800
Phe Lys Val Glu Leu Leu Asn Leu Glu Gly Gly Val Ala Glu Leu
1805 1810 1815
Phe Arg Val Asp Gly Ser Gly Ser Gly Asp Gly Asp Met Glu Phe
1820 1825 1830
Phe Leu Pro Thr Ile His Lys Arg Ala Ser Leu Gly Val Ala Ser
1835 1840 1845
Gln Ile Leu Val Thr Ile Ala Ala Ser Asp His Ala His Gly Val
1850 1855 1860
Phe Glu Phe Ser Pro Glu Ser Leu Phe Val Ser Gly Thr Glu Pro
1865 1870 1875
Glu Asp Gly Tyr Ser Thr Val Thr Leu Asn Val Ile Arg His His
1880 1885 1890
Gly Thr Leu Ser Pro Val Thr Leu His Trp Asn Ile Asp Ser Asp
1895 1900 1905
Pro Asp Gly Asp Leu Ala Phe Thr Ser Gly Asn Ile Thr Phe Glu
1910 1915 1920
Ile Gly Gln Thr Ser Ala Asn Ile Thr Val Glu Ile Leu Pro Asp
1925 1930 1935
Glu Asp Pro Glu Leu Asp Lys Ala Phe Ser Val Ser Val Leu Ser
1940 1945 1950
Val Ser Ser Gly Ser Leu Gly Ala His Ile Asn Ala Thr Leu Thr
1955 1960 1965
Val Leu Ala Ser Asp Asp Pro Tyr Gly Ile Phe Ile Phe Ser Glu
1970 1975 1980
Lys Asn Arg Pro Val Lys Val Glu Glu Ala Thr Gln Asn Ile Thr
1985 1990 1995
Leu Ser Ile Ile Arg Leu Lys Gly Leu Met Gly Lys Val Leu Val
2000 2005 2010
Ser Tyr Ala Thr Leu Asp Asp Met Glu Lys Pro Pro Tyr Phe Pro
2015 2020 2025
Pro Asn Leu Ala Arg Ala Thr Gln Gly Arg Asp Tyr Ile Pro Ala
2030 2035 2040
Ser Gly Phe Ala Leu Phe Gly Ala Asn Gln Ser Glu Ala Thr Ile
2045 2050 2055
Ala Ile Ser Ile Leu Asp Asp Asp Glu Pro Glu Arg Ser Glu Ser
2060 2065 2070
Val Phe Ile Glu Leu Leu Asn Ser Thr Leu Val Ala Lys Val Gln
2075 2080 2085
Ser Arg Ser Ile Pro Asn Ser Pro Arg Leu Gly Pro Lys Val Glu
2090 2095 2100
Thr Ile Ala Gln Leu Ile Ile Ile Ala Asn Asp Asp Ala Phe Gly
2105 2110 2115
Thr Leu Gln Leu Ser Ala Pro Ile Val Arg Val Ala Glu Asn His
2120 2125 2130
Val Gly Pro Ile Ile Asn Val Thr Arg Thr Gly Gly Ala Phe Ala
2135 2140 2145
Asp Val Ser Val Lys Phe Lys Ala Val Pro Ile Thr Ala Ile Ala
2150 2155 2160
Gly Glu Asp Tyr Ser Ile Ala Ser Ser Asp Val Val Leu Leu Glu
2165 2170 2175
Gly Glu Thr Ser Lys Ala Val Pro Ile Tyr Val Ile Asn Asp Ile
2180 2185 2190
Tyr Pro Glu Leu Glu Glu Ser Phe Leu Val Gln Leu Met Asn Glu
2195 2200 2205
Thr Thr Gly Gly Ala Arg Leu Gly Ala Leu Thr Glu Ala Val Ile
2210 2215 2220
Ile Ile Glu Ala Ser Asp Asp Pro Tyr Gly Leu Phe Gly Phe Gln
2225 2230 2235
Ile Thr Lys Leu Ile Val Glu Glu Pro Glu Phe Asn Ser Val Lys
2240 2245 2250
Val Asn Leu Pro Ile Ile Arg Asn Ser Gly Thr Leu Gly Asn Val
2255 2260 2265
Thr Val Arg Trp Val Ala Thr Ile Asn Gly Gln Leu Ala Thr Gly
2270 2275 2280
Asp Leu Arg Val Val Ser Gly Asn Val Thr Phe Ala Pro Gly Glu
2285 2290 2295
Thr Ile Gln Thr Leu Leu Leu Glu Val Leu Ala Asp Asp Val Pro
2300 2305 2310
Glu Ile Glu Glu Val Ile Gln Val Gln Leu Thr Asp Ala Ser Gly
2315 2320 2325
Gly Gly Thr Ile Gly Leu Asp Arg Ile Ala Asn Ile Ile Ile Pro
2330 2335 2340
Ala Asn Asp Asp Pro Tyr Gly Thr Val Ala Phe Ala Gln Met Val
2345 2350 2355
Tyr Arg Val Gln Glu Pro Leu Glu Arg Ser Ser Cys Ala Asn Ile
2360 2365 2370
Thr Val Arg Arg Ser Gly Gly His Phe Gly Arg Leu Leu Leu Phe
2375 2380 2385
Tyr Ser Thr Ser Asp Ile Asp Val Val Ala Leu Ala Met Glu Glu
2390 2395 2400
Gly Gln Asp Leu Leu Ser Tyr Tyr Glu Ser Pro Ile Gln Gly Val
2405 2410 2415
Pro Asp Pro Leu Trp Arg Thr Trp Met Asn Val Ser Ala Val Gly
2420 2425 2430
Glu Pro Leu Tyr Thr Cys Ala Thr Leu Cys Leu Lys Glu Gln Ala
2435 2440 2445
Cys Ser Ala Phe Ser Phe Phe Ser Ala Ser Glu Gly Pro Gln Cys
2450 2455 2460
Phe Trp Met Thr Ser Trp Ile Ser Pro Ala Val Asn Asn Ser Asp
2465 2470 2475
Phe Trp Thr Tyr Arg Lys Asn Met Thr Arg Val Ala Ser Leu Phe
2480 2485 2490
Ser Gly Gln Ala Val Ala Gly Ser Asp Tyr Glu Pro Val Thr Arg
2495 2500 2505
Gln Trp Ala Ile Met Gln Glu Gly Asp Glu Phe Ala Asn Leu Thr
2510 2515 2520
Val Ser Ile Leu Pro Asp Asp Phe Pro Glu Met Asp Glu Ser Phe
2525 2530 2535
Leu Ile Ser Leu Leu Glu Val His Leu Met Asn Ile Ser Ala Ser
2540 2545 2550
Leu Lys Asn Gln Pro Thr Ile Gly Gln Pro Asn Ile Ser Thr Val
2555 2560 2565
Val Ile Ala Leu Asn Gly Asp Ala Phe Gly Val Phe Val Ile Tyr
2570 2575 2580
Asn Ile Ser Pro Asn Thr Ser Glu Asp Gly Leu Phe Val Glu Val
2585 2590 2595
Gln Glu Gln Pro Gln Thr Leu Val Glu Leu Met Ile His Arg Thr
2600 2605 2610
Gly Gly Ser Leu Gly Gln Val Ala Val Glu Trp Arg Val Val Gly
2615 2620 2625
Gly Thr Ala Thr Glu Gly Leu Asp Phe Ile Gly Ala Gly Glu Ile
2630 2635 2640
Leu Thr Phe Ala Glu Gly Glu Thr Lys Lys Thr Val Ile Leu Thr
2645 2650 2655
Ile Leu Asp Asp Ser Glu Pro Glu Asp Asp Glu Ser Ile Ile Val
2660 2665 2670
Ser Leu Val Tyr Thr Glu Gly Gly Ser Arg Ile Leu Pro Ser Ser
2675 2680 2685
Asp Thr Val Arg Val Asn Ile Leu Ala Asn Asp Asn Val Ala Gly
2690 2695 2700
Ile Val Ser Phe Gln Thr Ala Ser Arg Ser Val Ile Gly His Glu
2705 2710 2715
Gly Glu Ile Leu Gln Phe His Val Ile Arg Thr Phe Pro Gly Arg
2720 2725 2730
Gly Asn Val Thr Val Asn Trp Lys Ile Ile Gly Gln Asn Leu Glu
2735 2740 2745
Leu Asn Phe Ala Asn Phe Ser Gly Gln Leu Phe Phe Pro Glu Gly
2750 2755 2760
Ser Leu Asn Thr Thr Leu Phe Val His Leu Leu Asp Asp Asn Ile
2765 2770 2775
Pro Glu Glu Lys Glu Val Tyr Gln Val Ile Leu Tyr Asp Val Arg
2780 2785 2790
Thr Gln Gly Val Pro Pro Ala Gly Ile Ala Leu Leu Asp Ala Gln
2795 2800 2805
Gly Tyr Ala Ala Val Leu Thr Val Glu Ala Ser Asp Glu Pro His
2810 2815 2820
Gly Val Leu Asn Phe Ala Leu Ser Ser Arg Phe Val Leu Leu Gln
2825 2830 2835
Glu Ala Asn Ile Thr Ile Gln Leu Phe Ile Asn Arg Glu Phe Gly
2840 2845 2850
Ser Leu Gly Ala Ile Asn Val Thr Tyr Thr Thr Val Pro Gly Met
2855 2860 2865
Leu Ser Leu Lys Asn Gln Thr Val Gly Asn Leu Ala Glu Pro Glu
2870 2875 2880
Val Asp Phe Val Pro Ile Ile Gly Phe Leu Ile Leu Glu Glu Gly
2885 2890 2895
Glu Thr Ala Ala Ala Ile Asn Ile Thr Ile Leu Glu Asp Asp Val
2900 2905 2910
Pro Glu Leu Glu Glu Tyr Phe Leu Val Asn Leu Thr Tyr Val Gly
2915 2920 2925
Leu Thr Met Ala Ala Ser Thr Ser Phe Pro Pro Arg Leu Asp Ser
2930 2935 2940
Glu Gly Leu Thr Ala Gln Val Ile Ile Asp Ala Asn Asp Gly Ala
2945 2950 2955
Arg Gly Val Ile Glu Trp Gln Gln Ser Arg Phe Glu Val Asn Glu
2960 2965 2970
Thr His Gly Ser Leu Thr Leu Val Ala Gln Arg Ser Arg Glu Pro
2975 2980 2985
Leu Gly His Val Ser Leu Phe Val Tyr Ala Gln Asn Leu Glu Ala
2990 2995 3000
Gln Val Gly Leu Asp Tyr Ile Phe Thr Pro Met Ile Leu His Phe
3005 3010 3015
Ala Asp Gly Glu Arg Tyr Lys Asn Val Asn Ile Met Ile Leu Asp
3020 3025 3030
Asp Asp Ile Pro Glu Gly Asp Glu Lys Phe Gln Leu Ile Leu Thr
3035 3040 3045
Asn Pro Ser Pro Gly Leu Glu Leu Gly Lys Asn Thr Ile Ala Leu
3050 3055 3060
Ile Ile Val Leu Ala Asn Asp Asp Gly Pro Gly Val Leu Ser Phe
3065 3070 3075
Asn Asn Ser Glu His Phe Phe Leu Arg Glu Pro Thr Ala Leu Tyr
3080 3085 3090
Val Gln Glu Ser Val Ala Val Leu Tyr Ile Val Arg Glu Pro Ala
3095 3100 3105
Gln Gly Leu Phe Gly Thr Val Thr Val Gln Phe Ile Val Thr Glu
3110 3115 3120
Val Asn Ser Ser Asn Glu Ser Lys Asp Leu Thr Pro Ser Lys Gly
3125 3130 3135
Tyr Ile Val Leu Glu Glu Gly Val Arg Phe Lys Ala Leu Gln Ile
3140 3145 3150
Ser Ala Ile Leu Asp Thr Glu Pro Glu Met Asp Glu Tyr Phe Val
3155 3160 3165
Cys Thr Leu Phe Asn Pro Thr Gly Gly Ala Arg Leu Gly Val His
3170 3175 3180
Val Gln Thr Leu Ile Thr Val Leu Gln Asn Gln Ala Pro Leu Gly
3185 3190 3195
Leu Phe Ser Ile Ser Ala Val Glu Asn Arg Ala Thr Ser Ile Asp
3200 3205 3210
Ile Glu Glu Ala Asn Arg Thr Val Tyr Leu Asn Val Ser Arg Thr
3215 3220 3225
Asn Gly Ile Asp Leu Ala Val Ser Val Gln Trp Glu Thr Val Ser
3230 3235 3240
Glu Thr Ala Phe Gly Met Arg Gly Met Asp Val Val Phe Ser Val
3245 3250 3255
Phe Gln Ser Phe Leu Asp Glu Ser Ala Ser Gly Trp Cys Phe Phe
3260 3265 3270
Thr Leu Glu Asn Leu Ile Tyr Gly Ile Met Leu Arg Lys Ser Ser
3275 3280 3285
Val Thr Val Tyr Arg Trp Gln Gly Ile Phe Ile Pro Val Glu Asp
3290 3295 3300
Leu Asn Ile Glu Asn Pro Lys Thr Cys Glu Ala Phe Asn Ile Gly
3305 3310 3315
Phe Ser Pro Tyr Phe Val Ile Thr His Glu Glu Arg Asn Glu Glu
3320 3325 3330
Lys Pro Ser Leu Asn Ser Val Phe Thr Phe Thr Ser Gly Phe Lys
3335 3340 3345
Leu Phe Leu Val Gln Thr Ile Ile Ile Leu Glu Ser Ser Gln Val
3350 3355 3360
Arg Tyr Phe Thr Ser Asp Ser Gln Asp Tyr Leu Ile Ile Ala Ser
3365 3370 3375
Gln Arg Asp Asp Ser Glu Leu Thr Gln Val Phe Arg Trp Asn Gly
3380 3385 3390
Gly Ser Phe Val Leu His Gln Lys Leu Pro Val Arg Gly Val Leu
3395 3400 3405
Thr Val Ala Leu Phe Asn Lys Gly Gly Ser Val Phe Leu Ala Ile
3410 3415 3420
Ser Gln Ala Asn Ala Arg Leu Asn Ser Leu Leu Phe Arg Trp Ser
3425 3430 3435
Gly Ser Gly Phe Ile Asn Phe Gln Glu Val Pro Val Ser Gly Thr
3440 3445 3450
Thr Glu Val Glu Ala Leu Ser Ser Ala Asn Asp Ile Tyr Leu Ile
3455 3460 3465
Phe Ala Glu Asn Val Phe Leu Gly Asp Gln Asn Ser Ile Asp Ile
3470 3475 3480
Phe Ile Trp Glu Met Gly Gln Ser Ser Phe Arg Tyr Phe Gln Ser
3485 3490 3495
Val Asp Phe Ala Ala Val Asn Arg Ile His Ser Phe Thr Pro Ala
3500 3505 3510
Ser Gly Ile Ala His Ile Leu Leu Ile Gly Gln Asp Met Ser Ala
3515 3520 3525
Leu Tyr Cys Trp Asn Ser Glu Arg Asn Gln Phe Ser Phe Val Leu
3530 3535 3540
Glu Val Pro Ser Ala Tyr Asp Val Ala Ser Val Thr Val Lys Ser
3545 3550 3555
Leu Asn Ser Ser Lys Asn Leu Ile Ala Leu Val Gly Ala His Ser
3560 3565 3570
His Ile Tyr Glu Leu Ala Tyr Ile Ser Ser His Ser Asp Phe Ile
3575 3580 3585
Pro Ser Ser Gly Glu Leu Ile Phe Glu Pro Gly Glu Arg Glu Ala
3590 3595 3600
Thr Ile Ala Val Asn Ile Leu Asp Asp Thr Val Pro Glu Lys Glu
3605 3610 3615
Glu Ser Phe Lys Val Gln Leu Lys Asn Pro Lys Gly Gly Ala Glu
3620 3625 3630
Ile Gly Ile Asn Asp Ser Val Thr Ile Thr Ile Leu Ser Asn Asp
3635 3640 3645
Asp Ala Tyr Gly Ile Val Ala Phe Ala Gln Asn Ser Leu Tyr Lys
3650 3655 3660
Gln Val Glu Glu Met Glu Gln Asp Ser Leu Val Thr Leu Asn Val
3665 3670 3675
Glu Arg Leu Lys Gly Thr Tyr Gly Arg Ile Thr Ile Ala Trp Glu
3680 3685 3690
Ala Asp Gly Ser Ile Ser Asp Ile Phe Pro Thr Ser Gly Val Ile
3695 3700 3705
Leu Phe Thr Glu Gly Gln Val Leu Ser Thr Ile Thr Leu Thr Ile
3710 3715 3720
Leu Ala Asp Asn Ile Pro Glu Leu Ser Glu Val Val Ile Val Thr
3725 3730 3735
Leu Thr Arg Ile Thr Thr Glu Gly Val Glu Asp Ser Tyr Lys Gly
3740 3745 3750
Ala Thr Ile Asp Gln Asp Arg Ser Lys Ser Val Ile Thr Thr Leu
3755 3760 3765
Pro Asn Asp Ser Pro Phe Gly Leu Val Gly Trp Arg Ala Ala Ser
3770 3775 3780
Val Phe Ile Arg Val Ala Glu Pro Lys Glu Asn Thr Thr Thr Leu
3785 3790 3795
Gln Leu Gln Ile Ala Arg Asp Lys Gly Leu Leu Gly Asp Ile Ala
3800 3805 3810
Ile His Leu Arg Ala Gln Pro Asn Phe Leu Leu His Val Asp Asn
3815 3820 3825
Gln Ala Thr Glu Asn Glu Asp Tyr Val Leu Gln Glu Thr Ile Ile
3830 3835 3840
Ile Met Lys Glu Asn Ile Lys Glu Ala His Ala Glu Val Ser Ile
3845 3850 3855
Leu Pro Asp Asp Leu Pro Glu Leu Glu Glu Gly Phe Ile Val Thr
3860 3865 3870
Ile Thr Glu Val Asn Leu Val Asn Ser Asp Phe Ser Thr Gly Gln
3875 3880 3885
Pro Ser Val Arg Arg Pro Gly Met Glu Ile Ala Glu Ile Met Ile
3890 3895 3900
Glu Glu Asn Asp Asp Pro Arg Gly Ile Phe Met Phe His Val Thr
3905 3910 3915
Arg Gly Ala Gly Glu Val Ile Thr Ala Tyr Glu Val Pro Pro Pro
3920 3925 3930
Leu Asn Val Leu Gln Val Pro Val Val Arg Leu Ala Gly Ser Phe
3935 3940 3945
Gly Ala Val Asn Val Tyr Trp Lys Ala Ser Pro Asp Ser Ala Gly
3950 3955 3960
Leu Glu Asp Phe Lys Pro Ser His Gly Ile Leu Glu Phe Ala Asp
3965 3970 3975
Lys Gln Val Thr Ala Met Ile Glu Ile Thr Ile Ile Asp Asp Ala
3980 3985 3990
Glu Phe Glu Leu Thr Glu Thr Phe Asn Ile Ser Leu Ile Ser Val
3995 4000 4005
Ala Gly Gly Gly Arg Leu Gly Asp Asp Val Val Val Thr Val Val
4010 4015 4020
Ile Pro Gln Asn Asp Ser Pro Phe Gly Val Phe Gly Phe Glu Glu
4025 4030 4035
Lys Thr Val Met Ile Asp Glu Ser Leu Ser Ser Asp Asp Pro Asp
4040 4045 4050
Ser Tyr Val Thr Leu Thr Val Val Arg Ser Pro Gly Gly Lys Gly
4055 4060 4065
Thr Val Arg Leu Glu Trp Thr Ile Asp Glu Lys Ala Lys His Asn
4070 4075 4080
Leu Ser Pro Leu Asn Gly Thr Leu His Phe Asp Glu Thr Glu Ser
4085 4090 4095
Gln Lys Thr Ile Val Leu His Thr Leu Gln Asp Thr Val Leu Glu
4100 4105 4110
Glu Asp Arg Arg Phe Thr Ile Gln Leu Ile Ser Ile Asp Glu Val
4115 4120 4125
Glu Ile Ser Pro Val Lys Gly Ser Ala Ser Ile Ile Ile Arg Gly
4130 4135 4140
Asp Lys Arg Ala Ser Gly Glu Val Gly Ile Ala Pro Ser Ser Arg
4145 4150 4155
His Ile Leu Ile Gly Glu Pro Ser Ala Lys Tyr Asn Gly Thr Ala
4160 4165 4170
Ile Ile Ser Leu Val Arg Gly Pro Gly Ile Leu Gly Glu Val Thr
4175 4180 4185
Val Phe Trp Arg Ile Phe Pro Pro Ser Val Gly Glu Phe Ala Glu
4190 4195 4200
Thr Ser Gly Lys Leu Thr Met Arg Asp Glu Gln Ser Ala Val Ile
4205 4210 4215
Val Val Ile Gln Ala Leu Asn Asp Asp Ile Pro Glu Glu Lys Ser
4220 4225 4230
Phe Tyr Glu Phe Gln Leu Thr Ala Val Ser Glu Gly Gly Val Leu
4235 4240 4245
Ser Glu Ser Ser Ser Thr Ala Asn Ile Thr Val Val Ala Ser Asp
4250 4255 4260
Ser Pro Tyr Gly Arg Phe Ala Phe Ser His Glu Gln Leu Arg Val
4265 4270 4275
Ser Glu Ala Gln Arg Val Asn Ile Thr Ile Ile Arg Ser Ser Gly
4280 4285 4290
Asp Phe Gly His Val Arg Leu Trp Tyr Lys Thr Met Ser Gly Thr
4295 4300 4305
Ala Glu Ala Gly Leu Asp Phe Val Pro Ala Ala Gly Glu Leu Leu
4310 4315 4320
Phe Glu Ala Gly Glu Met Arg Lys Ser Leu His Val Glu Ile Leu
4325 4330 4335
Asp Asp Asp Tyr Pro Glu Gly Pro Glu Glu Phe Ser Leu Thr Ile
4340 4345 4350
Thr Lys Val Glu Leu Gln Gly Arg Gly Tyr Asp Phe Thr Ile Gln
4355 4360 4365
Glu Asn Gly Leu Gln Ile Asp Gln Pro Pro Glu Ile Gly Asn Ile
4370 4375 4380
Ser Ile Val Arg Ile Ile Ile Met Lys Asn Asp Asn Ala Glu Gly
4385 4390 4395
Ile Ile Glu Phe Asp Pro Lys Tyr Thr Ala Phe Glu Val Glu Glu
4400 4405 4410
Asp Val Gly Leu Ile Met Ile Pro Val Val Arg Leu His Gly Thr
4415 4420 4425
Tyr Gly Tyr Val Thr Ala Asp Phe Ile Ser Gln Ser Ser Ser Ala
4430 4435 4440
Ser Pro Gly Gly Val Asp Tyr Ile Leu His Gly Ser Thr Val Thr
4445 4450 4455
Phe Gln His Gly Gln Asn Leu Ser Phe Ile Asn Ile Ser Ile Ile
4460 4465 4470
Asp Asp Asn Glu Ser Glu Phe Glu Glu Pro Ile Glu Ile Leu Leu
4475 4480 4485
Thr Gly Ala Thr Gly Gly Ala Val Leu Gly Arg His Leu Val Ser
4490 4495 4500
Arg Ile Ile Ile Ala Lys Ser Asp Ser Pro Phe Gly Val Ile Arg
4505 4510 4515
Phe Leu Asn Gln Ser Lys Ile Ser Ile Ala Asn Pro Asn Ser Thr
4520 4525 4530
Met Ile Leu Ser Leu Val Leu Glu Arg Thr Gly Gly Leu Leu Gly
4535 4540 4545
Glu Ile Gln Val Asn Trp Glu Thr Val Gly Pro Asn Ser Gln Glu
4550 4555 4560
Ala Leu Leu Pro Gln Asn Arg Asp Ile Ala Asp Pro Val Ser Gly
4565 4570 4575
Leu Phe Tyr Phe Gly Glu Gly Glu Gly Gly Val Arg Thr Ile Ile
4580 4585 4590
Leu Thr Ile Tyr Pro His Glu Glu Ile Glu Val Glu Glu Thr Phe
4595 4600 4605
Ile Ile Lys Leu His Leu Val Lys Gly Glu Ala Lys Leu Asp Ser
4610 4615 4620
Arg Ala Lys Asp Val Thr Leu Thr Ile Gln Glu Phe Gly Asp Pro
4625 4630 4635
Asn Gly Val Val Gln Phe Ala Pro Glu Thr Leu Ser Lys Lys Thr
4640 4645 4650
Tyr Ser Glu Pro Leu Ala Leu Glu Gly Pro Leu Leu Ile Thr Phe
4655 4660 4665
Phe Val Arg Arg Val Lys Gly Thr Phe Gly Glu Ile Met Val Tyr
4670 4675 4680
Trp Glu Leu Ser Ser Glu Phe Asp Ile Thr Glu Asp Phe Leu Ser
4685 4690 4695
Thr Ser Gly Phe Phe Thr Ile Ala Asp Gly Glu Ser Glu Ala Ser
4700 4705 4710
Phe Asp Val His Leu Leu Pro Asp Glu Val Pro Glu Ile Glu Glu
4715 4720 4725
Asp Tyr Val Ile Gln Leu Val Ser Val Glu Gly Gly Ala Glu Leu
4730 4735 4740
Asp Leu Glu Lys Ser Ile Thr Trp Phe Ser Val Tyr Ala Asn Asp
4745 4750 4755
Asp Pro His Gly Val Phe Ala Leu Tyr Ser Asp Arg Gln Ser Ile
4760 4765 4770
Leu Ile Gly Gln Asn Leu Ile Arg Ser Ile Gln Ile Asn Ile Thr
4775 4780 4785
Arg Leu Ala Gly Thr Phe Gly Asp Val Ala Val Gly Leu Arg Ile
4790 4795 4800
Ser Ser Asp His Lys Glu Gln Pro Ile Val Thr Glu Asn Ala Glu
4805 4810 4815
Arg Gln Leu Val Val Lys Asp Gly Ala Thr Tyr Lys Val Asp Val
4820 4825 4830
Val Pro Ile Lys Asn Gln Val Phe Leu Ser Leu Gly Ser Asn Phe
4835 4840 4845
Thr Leu Gln Leu Val Thr Val Met Leu Val Gly Gly Arg Phe Tyr
4850 4855 4860
Gly Met Pro Thr Ile Leu Gln Glu Ala Lys Ser Ala Val Leu Pro
4865 4870 4875
Val Ser Glu Lys Ala Ala Asn Ser Gln Val Gly Phe Glu Ser Thr
4880 4885 4890
Ala Phe Gln Leu Met Asn Ile Thr Ala Gly Thr Ser His Val Met
4895 4900 4905
Ile Ser Arg Arg Gly Thr Tyr Gly Ala Leu Ser Val Ala Trp Thr
4910 4915 4920
Thr Gly Tyr Ala Pro Gly Leu Glu Ile Pro Glu Phe Ile Val Val
4925 4930 4935
Gly Asn Met Thr Pro Thr Leu Gly Ser Leu Ser Phe Ser His Gly
4940 4945 4950
Glu Gln Arg Lys Gly Val Phe Leu Trp Thr Phe Pro Ser Pro Gly
4955 4960 4965
Trp Pro Glu Ala Phe Val Leu His Leu Ser Gly Val Gln Ser Ser
4970 4975 4980
Ala Pro Gly Gly Ala Gln Leu Arg Ser Gly Phe Ile Val Ala Glu
4985 4990 4995
Ile Glu Pro Met Gly Val Phe Gln Phe Ser Thr Ser Ser Arg Asn
5000 5005 5010
Ile Ile Val Ser Glu Asp Thr Gln Met Ile Arg Leu His Val Gln
5015 5020 5025
Arg Leu Phe Gly Phe His Ser Asp Leu Ile Lys Val Ser Tyr Gln
5030 5035 5040
Thr Thr Ala Gly Ser Ala Lys Pro Leu Glu Asp Phe Glu Pro Val
5045 5050 5055
Gln Asn Gly Glu Leu Phe Phe Gln Lys Phe Gln Thr Glu Val Asp
5060 5065 5070
Phe Glu Ile Thr Ile Ile Asn Asp Gln Leu Ser Glu Ile Glu Glu
5075 5080 5085
Phe Phe Tyr Ile Asn Leu Thr Ser Val Glu Ile Arg Gly Leu Gln
5090 5095 5100
Lys Phe Asp Val Asn Trp Ser Pro Arg Leu Asn Leu Asp Phe Ser
5105 5110 5115
Val Ala Val Ile Thr Ile Leu Asp Asn Asp Asp Leu Ala Gly Met
5120 5125 5130
Asp Ile Ser Phe Pro Glu Thr Thr Val Ala Val Ala Val Asp Thr
5135 5140 5145
Thr Leu Ile Pro Val Glu Thr Glu Ser Thr Thr Tyr Leu Ser Thr
5150 5155 5160
Ser Lys Thr Thr Thr Ile Leu Gln Pro Thr Asn Val Val Ala Ile
5165 5170 5175
Val Thr Glu Ala Thr Gly Val Ser Ala Ile Pro Glu Lys Leu Val
5180 5185 5190
Thr Leu His Gly Thr Pro Ala Val Ser Glu Lys Pro Asp Val Ala
5195 5200 5205
Thr Val Thr Ala Asn Val Ser Ile His Gly Thr Phe Ser Leu Gly
5210 5215 5220
Pro Ser Ile Val Tyr Ile Glu Glu Glu Met Lys Asn Gly Thr Phe
5225 5230 5235
Asn Thr Ala Glu Val Leu Ile Arg Arg Thr Gly Gly Phe Thr Gly
5240 5245 5250
Asn Val Ser Ile Thr Val Lys Thr Phe Gly Glu Arg Cys Ala Gln
5255 5260 5265
Met Glu Pro Asn Ala Leu Pro Phe Arg Gly Ile Tyr Gly Ile Ser
5270 5275 5280
Asn Leu Thr Trp Ala Val Glu Glu Glu Asp Phe Glu Glu Gln Thr
5285 5290 5295
Leu Thr Leu Ile Phe Leu Asp Gly Glu Arg Glu Arg Lys Val Ser
5300 5305 5310
Val Gln Ile Leu Asp Asp Asp Glu Pro Glu Gly Gln Glu Phe Phe
5315 5320 5325
Tyr Val Phe Leu Thr Asn Pro Gln Gly Gly Ala Gln Ile Val Glu
5330 5335 5340
Glu Lys Asp Asp Thr Gly Phe Ala Ala Phe Ala Met Val Ile Ile
5345 5350 5355
Thr Gly Ser Asp Leu His Asn Gly Ile Ile Gly Phe Ser Glu Glu
5360 5365 5370
Ser Gln Ser Gly Leu Glu Leu Arg Glu Gly Ala Val Met Arg Arg
5375 5380 5385
Leu His Leu Ile Val Thr Arg Gln Pro Asn Arg Ala Phe Glu Asp
5390 5395 5400
Val Lys Val Phe Trp Arg Val Thr Leu Asn Lys Thr Val Val Val
5405 5410 5415
Leu Gln Lys Asp Gly Val Asn Leu Val Glu Glu Leu Gln Ser Val
5420 5425 5430
Ser Gly Thr Thr Thr Cys Thr Met Gly Gln Thr Lys Cys Phe Ile
5435 5440 5445
Ser Ile Glu Leu Lys Pro Glu Lys Val Pro Gln Val Glu Val Tyr
5450 5455 5460
Phe Phe Val Glu Leu Tyr Glu Ala Thr Ala Gly Ala Ala Ile Asn
5465 5470 5475
Asn Ser Ala Arg Phe Ala Gln Ile Lys Ile Leu Glu Ser Asp Glu
5480 5485 5490
Ser Gln Ser Leu Val Tyr Phe Ser Val Gly Ser Arg Leu Ala Val
5495 5500 5505
Ala His Lys Lys Ala Thr Leu Ile Ser Leu Gln Val Ala Arg Asp
5510 5515 5520
Ser Gly Thr Gly Leu Met Met Ser Val Asn Phe Ser Thr Gln Glu
5525 5530 5535
Leu Arg Ser Ala Glu Thr Ile Gly Arg Thr Ile Ile Ser Pro Ala
5540 5545 5550
Ile Ser Gly Lys Asp Phe Val Ile Thr Glu Gly Thr Leu Val Phe
5555 5560 5565
Glu Pro Gly Gln Arg Ser Thr Val Leu Asp Val Ile Leu Thr Pro
5570 5575 5580
Glu Thr Gly Ser Leu Asn Ser Phe Pro Lys Arg Phe Gln Ile Val
5585 5590 5595
Leu Phe Asp Pro Lys Gly Gly Ala Arg Ile Asp Lys Val Tyr Gly
5600 5605 5610
Thr Ala Asn Ile Thr Leu Val Ser Asp Ala Asp Ser Gln Ala Ile
5615 5620 5625
Trp Gly Leu Ala Asp Gln Leu His Gln Pro Val Asn Asp Asp Ile
5630 5635 5640
Leu Asn Arg Val Leu His Thr Ile Ser Met Lys Val Ala Thr Glu
5645 5650 5655
Asn Thr Asp Glu Gln Leu Ser Ala Met Met His Leu Ile Glu Lys
5660 5665 5670
Ile Thr Thr Glu Gly Lys Ile Gln Ala Phe Ser Val Ala Ser Arg
5675 5680 5685
Thr Leu Phe Tyr Glu Ile Leu Cys Ser Leu Ile Asn Pro Lys Arg
5690 5695 5700
Lys Asp Thr Arg Gly Phe Ser His Phe Ala Glu Val Thr Glu Asn
5705 5710 5715
Phe Ala Phe Ser Leu Leu Thr Asn Val Thr Cys Gly Ser Pro Gly
5720 5725 5730
Glu Lys Ser Lys Thr Ile Leu Asp Ser Cys Pro Tyr Leu Ser Ile
5735 5740 5745
Leu Ala Leu His Trp Tyr Pro Gln Gln Ile Asn Gly His Lys Phe
5750 5755 5760
Glu Gly Lys Glu Gly Asp Tyr Ile Arg Ile Pro Glu Arg Leu Leu
5765 5770 5775
Asp Val Gln Asp Ala Glu Ile Met Ala Gly Lys Ser Thr Cys Lys
5780 5785 5790
Leu Val Gln Phe Thr Glu Tyr Ser Ser Gln Gln Trp Phe Ile Ser
5795 5800 5805
Gly Asn Asn Leu Pro Thr Leu Lys Asn Lys Val Leu Ser Leu Ser
5810 5815 5820
Val Lys Gly Gln Ser Ser Gln Leu Leu Thr Asn Asp Asn Glu Val
5825 5830 5835
Leu Tyr Arg Ile Tyr Ala Ala Glu Pro Arg Ile Ile Pro Gln Thr
5840 5845 5850
Ser Leu Cys Leu Leu Trp Asn Gln Ala Ala Ala Ser Trp Leu Ser
5855 5860 5865
Asp Ser Gln Phe Cys Lys Val Val Glu Glu Thr Ala Asp Tyr Val
5870 5875 5880
Glu Cys Ala Cys Ser His Met Ser Val Tyr Ala Val Tyr Ala Arg
5885 5890 5895
Thr Asp Asn Leu Ser Ser Tyr Asn Glu Ala Phe Phe Thr Ser Gly
5900 5905 5910
Phe Ile Cys Ile Ser Gly Leu Cys Leu Ala Val Leu Ser His Ile
5915 5920 5925
Phe Cys Ala Arg Tyr Ser Met Phe Ala Ala Lys Leu Leu Thr His
5930 5935 5940
Met Met Ala Ala Ser Leu Gly Thr Gln Ile Leu Phe Leu Ala Ser
5945 5950 5955
Ala Tyr Ala Ser Pro Gln Leu Ala Glu Glu Ser Cys Ser Ala Met
5960 5965 5970
Ala Ala Val Thr His Tyr Leu Tyr Leu Cys Gln Phe Ser Trp Met
5975 5980 5985
Leu Ile Gln Ser Val Asn Phe Trp Tyr Val Leu Val Met Asn Asp
5990 5995 6000
Glu His Thr Glu Arg Arg Tyr Leu Leu Phe Phe Leu Leu Ser Trp
6005 6010 6015
Gly Leu Pro Ala Phe Val Val Ile Leu Leu Ile Val Ile Leu Lys
6020 6025 6030
Gly Ile Tyr His Gln Ser Met Ser Gln Ile Tyr Gly Leu Ile His
6035 6040 6045
Gly Asp Leu Cys Phe Ile Pro Asn Val Tyr Ala Ala Leu Phe Thr
6050 6055 6060
Ala Ala Leu Val Pro Leu Thr Cys Leu Val Val Val Phe Val Val
6065 6070 6075
Phe Ile His Ala Tyr Gln Val Lys Pro Gln Trp Lys Ala Tyr Asp
6080 6085 6090
Asp Val Phe Arg Gly Arg Thr Asn Ala Ala Glu Ile Pro Leu Ile
6095 6100 6105
Leu Tyr Leu Phe Ala Leu Ile Ser Val Thr Trp Leu Trp Gly Gly
6110 6115 6120
Leu His Met Ala Tyr Arg His Phe Trp Met Leu Val Leu Phe Val
6125 6130 6135
Ile Phe Asn Ser Leu Gln Gly Leu Tyr Val Phe Met Val Tyr Phe
6140 6145 6150
Ile Leu His Asn Gln Met Cys Cys Pro Met Lys Ala Ser Tyr Thr
6155 6160 6165
Val Glu Met Asn Gly His Pro Gly Pro Ser Thr Ala Phe Phe Thr
6170 6175 6180
Pro Gly Ser Gly Met Pro Pro Ala Gly Gly Glu Ile Ser Lys Ser
6185 6190 6195
Thr Gln Asn Leu Ile Gly Ala Met Glu Glu Val Pro Pro Asp Trp
6200 6205 6210
Glu Arg Ala Ser Phe Gln Gln Gly Ser Gln Ala Ser Pro Asp Leu
6215 6220 6225
Lys Pro Ser Pro Gln Asn Gly Ala Thr Phe Pro Ser Ser Gly Gly
6230 6235 6240
Tyr Gly Gln Gly Ser Leu Ile Ala Asp Glu Glu Ser Gln Glu Phe
6245 6250 6255
Asp Asp Leu Ile Phe Ala Leu Lys Thr Gly Ala Gly Leu Ser Val
6260 6265 6270
Ser Asp Asn Glu Ser Gly Gln Gly Ser Gln Glu Gly Gly Thr Leu
6275 6280 6285
Thr Asp Ser Gln Ile Val Glu Leu Arg Arg Ile Pro Ile Ala Asp
6290 6295 6300
Thr His Leu
6305